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trnH-GUG-psbA

LSC · 157-583 · 427 bp

Back to Tapeinosperma

Pi 0.00703

MarkerSeek score 59.20

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 427
variable_sites: 3
pi: 0.00703
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	TTAGTTATGGGCGAACGACG	TTCCCTCTAGACCTAGCTGC	50.0	55.0	58.1	57.9	558 bp	1.0	79.1
trnH-GUG-psbA_p2	CTGCCTTAATCCACTTGGCT	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	503 bp	1.0	78.8
trnH-GUG-psbA_p3	ACTGCCTTAATCCACTTGGC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	58.2	57.9	504 bp	1.0	78.6
trnH-GUG-psbA_p4	AATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.7	57.9	496 bp	1.0	77.2
trnH-GUG-psbA_p5	TTAGTTATGGGCGAACGACG	TAGACCTAGCTGCGGTTGAA	50.0	50.0	58.1	58.4	551 bp	1.0	76.2