Hotspot

ycf4

LSC · 60017-60538 · 522 bp

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Pi 0.0664

MarkerSeek score 74.80

Primer available yes

Label ycf4

Feature Metadata

label_name: ycf4
feature_type: gene
region: LSC
length_bp: 522
variable_sites: 34
pi: 0.0664
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4_p1	TAGGCCTAGTATTTCCGGCA	TTGCATTTTTCCCTCCTGCT	50.0	45.0	57.9	58.0	1127-1180 bp	1.0	87.6
ycf4_p2	AAAACAGGAGCAGATGCCAA	TTGCATTTTTCCCTCCTGCT	45.0	45.0	58.0	58.0	652 bp	0.5	59.9
ycf4_p3	AAAACAGGAGCAGATGCCAA	TGGCTCGACTGAATGTTCTG	45.0	50.0	58.0	57.9	715 bp	0.5	59.3
ycf4_p4	AAAACAGGAGCAGATGCCAA	ACAAGACAGAAATGGGCCAG	45.0	50.0	58.0	58.1	1044 bp	0.5	59.3
ycf4_p5	AAAACAGGAGCAGATGCCAA	CAAGACAGAAATGGGCCAGT	45.0	50.0	58.0	58.1	1043 bp	0.5	59.3