Hotspot

rpoA

LSC · 79315-80445 · 1131 bp

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Pi 0.114

MarkerSeek score 81.90

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1131
variable_sites: 115
pi: 0.114
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	TTGGTTGGGTATTGGTGCAA	CGCCTTTGTACGGGATGTAA	45.0	50.0	57.9	58.0	1427-1562 bp	1.0	90.6
rpoA_p2	TTTGGTTGGGTATTGGTGCA	CGCCTTTGTACGGGATGTAA	45.0	50.0	57.9	58.0	1428-1563 bp	1.0	90.6
rpoA_p3	CAAAATCCATTTCGCCGTCC	CGCCTTTGTACGGGATGTAA	50.0	50.0	58.1	58.0	1489-1624 bp	1.0	90.3
rpoA_p4	CCAAAATCCATTTCGCCGTC	CGCCTTTGTACGGGATGTAA	50.0	50.0	58.1	58.0	1490-1625 bp	1.0	90.3
rpoA_p5	TTGGTTGGGTATTGGTGCAA	AGGGTACGAGAAAAGGGACA	45.0	50.0	57.9	58.0	1590-1725 bp	1.0	89.8