Hotspot

clpP

LSC · 71365-73406 · 2042 bp

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Pi 0.0383

MarkerSeek score 69.70

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2042
variable_sites: 76
pi: 0.0383
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TCATTCATACCGGAAAGCGG	ATACCAAGGCAAACCCATGG	50.0	50.0	58.1	58.1	2879-2992 bp	1.0	82.6
clpP_p2	AGTCGCACATACACCCTAGT	ATACCAAGGCAAACCCATGG	50.0	50.0	58.2	58.1	2820-2933 bp	1.0	81.9
clpP_p3	TCGAATTGGCTGTCTTGCAT	TTCGTTACAGTCCCTCCTGT	45.0	50.0	58.2	58.0	2988 bp	0.5	58.6
clpP_p4	AGTCGCACATACACCCTAGT	ACGAACATACCCTGTCTCCA	50.0	50.0	58.2	58.1	2987 bp	0.5	58.3
clpP_p5	TCGAATTGGCTGTCTTGCAT	ACGAACATACCCTGTCTCCA	45.0	50.0	58.2	58.1	2913 bp	0.5	58.1