Hotspot

ycf1-trnN-GUU

IRa · 135484-136309 · 826 bp

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Pi 0.04

MarkerSeek score 71.60

Primer available yes

Label ycf1-trnN-GUU

Feature Metadata

label_name: ycf1-trnN-GUU
feature_type: igs
region: IRa
length_bp: 826
variable_sites: 24
pi: 0.04
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.73
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnN-GUU_p1	ACGACAACGACCGAATTGAT	AGGATTCGAACCTACGACCA	45.0	50.0	57.9	58.2	740-965 bp	1.0	84.4
ycf1-trnN-GUU_p2	ACGACAACGACCGAATTGAT	CCCCAAGTAGGATTCGAACC	45.0	55.0	57.9	57.7	748-973 bp	1.0	83.0
ycf1-trnN-GUU_p3	TCCATAATAGAGCCCGACGA	AGGATTCGAACCTACGACCA	50.0	50.0	57.7	58.2	756-981 bp	1.0	82.7
ycf1-trnN-GUU_p4	CCATAATAGAGCCCGACGAC	AGGATTCGAACCTACGACCA	55.0	50.0	57.7	58.2	755-980 bp	1.0	82.2
ycf1-trnN-GUU_p5	TCCATAATAGAGCCCGACGA	CCCCAAGTAGGATTCGAACC	50.0	55.0	57.7	57.7	764-989 bp	1.0	81.3