Hotspot

rpoA-rps11

LSC · 80446-80516 · 71 bp

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Pi 0.23

MarkerSeek score 75.10

Primer available yes

Label rpoA-rps11

Feature Metadata

label_name: rpoA-rps11
feature_type: igs
region: LSC
length_bp: 71
variable_sites: 14
pi: 0.23
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA-rps11_p1	AGTGGAGCCAGAATAAAGCG	CGCCTTTGTACGGGATGTAA	50.0	50.0	58.0	58.0	251 bp	1.0	90.8
rpoA-rps11_p2	AGTGGAGCCAGAATAAAGCG	GATTCGCCTTTGTACGGGAT	50.0	50.0	58.0	58.1	255 bp	1.0	90.5
rpoA-rps11_p3	AGTGGAGCCAGAATAAAGCG	AGGGTACGAGAAAAGGGACA	50.0	50.0	58.0	58.0	414 bp	1.0	87.7
rpoA-rps11_p4	AGTGGAGCCAGAATAAAGCG	CCGAGAACTGGTTCACGTAG	50.0	55.0	58.0	58.0	631 bp	1.0	85.2
rpoA-rps11_p5	AGTGGAGCCAGAATAAAGCG	CGAGAACTGGTTCACGTAGG	50.0	55.0	58.0	58.0	630 bp	1.0	85.2