Hotspot

psbZ-trnG-UCC

LSC · 35889-36153 · 265 bp

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Pi 0.0344

MarkerSeek score 69.20

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 265
variable_sites: 9
pi: 0.0344
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	TTGCTTCTCCTGATGGTTGG	TCGAACCCACATCTTCTCCT	50.0	50.0	57.8	58.1	431-458 bp	1.0	80.0
psbZ-trnG-UCC_p2	GCTTCTCCTGATGGTTGGTC	TCGAACCCACATCTTCTCCT	55.0	50.0	58.3	58.1	429-456 bp	1.0	79.6
psbZ-trnG-UCC_p3	CCGTCGTATTTGCTTCTCCT	TCGAACCCACATCTTCTCCT	50.0	50.0	57.7	58.1	440-467 bp	1.0	79.3
psbZ-trnG-UCC_p4	TTGCTTCTCCTGATGGTTGG	CCACATCTTCTCCTTGGCAA	50.0	50.0	57.8	57.8	425-452 bp	1.0	78.9
psbZ-trnG-UCC_p5	GCTTCTCCTGATGGTTGGTC	CCACATCTTCTCCTTGGCAA	55.0	50.0	58.3	57.8	423-450 bp	1.0	78.5