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psbK-psbI

LSC · 7827-8208 · 382 bp

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Pi 0.0191

MarkerSeek score 66.40

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 382
variable_sites: 7
pi: 0.0191
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	ACGCCCCGGATCATTAGATA	50.0	50.0	57.9	58.1	488-502 bp	1.0	82.6
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TACGCCCCGGATCATTAGAT	50.0	50.0	57.9	58.1	489-503 bp	1.0	82.6
psbK-psbI_p3	CTTTTGTTTGGCAAGCTGCT	ACGCCCCGGATCATTAGATA	45.0	50.0	58.1	58.1	493-507 bp	1.0	82.5
psbK-psbI_p4	CTTTTGTTTGGCAAGCTGCT	TACGCCCCGGATCATTAGAT	45.0	50.0	58.1	58.1	494-508 bp	1.0	82.5
psbK-psbI_p5	GCCAGTCATACCTGTGCTTT	ACGCCCCGGATCATTAGATA	50.0	50.0	58.2	58.1	525-539 bp	1.0	81.4