Hotspot

ycf1

SSC · 110478-116003 · 5526 bp

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Pi 0.0104

MarkerSeek score 47.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5526
variable_sites: 139
pi: 0.0104
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTTCCTTTTGGTTCTCCCCG	GAGCCAGGTTTCAATGTCGA	50.0	50.0	58.0	58.2	1473 bp	1.0	81.0
ycf1_p2	TTTCCTTTTGGTTCTCCCCG	TCGAGCCAGGTTTCAATGTC	50.0	50.0	58.0	58.2	1475 bp	1.0	81.0
ycf1_p3	TTTCCAGGGTCAATGCACAA	GAGCCAGGTTTCAATGTCGA	45.0	50.0	57.9	58.2	881 bp	1.0	80.5
ycf1_p4	CCTTTTGGTTCTCCCCGAAA	GAGCCAGGTTTCAATGTCGA	50.0	50.0	58.0	58.2	1470 bp	0.2	46.4
ycf1_p5	CCTTTTGGTTCTCCCCGAAA	TCGAGCCAGGTTTCAATGTC	50.0	50.0	58.0	58.2	1472 bp	0.2	46.4