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rpl16-rps3

LSC · 82539-83798 · 1260 bp

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Pi 0.00881

MarkerSeek score 57.80

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 1260
variable_sites: 27
pi: 0.00881
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	GTTCAAGTGCCTGAAGAGCA	TGCGCCTATACAGTTCGAAC	50.0	50.0	58.4	58.1	1450-1452 bp	1.0	77.6
rpl16-rps3_p2	GTTCAAGTGCCTGAAGAGCA	GCGCCTATACAGTTCGAACT	50.0	50.0	58.4	57.8	1449-1451 bp	1.0	76.6
rpl16-rps3_p3	AGGTTCAAGTGCCTGAAGAG	TGCGCCTATACAGTTCGAAC	50.0	50.0	57.4	58.1	1452-1454 bp	1.0	76.4
rpl16-rps3_p4	TTCAAGTGCCTGAAGAGCAT	TGCGCCTATACAGTTCGAAC	45.0	50.0	57.4	58.1	1449-1451 bp	1.0	76.2
rpl16-rps3_p5	AGGTTCAAGTGCCTGAAGAG	GCGCCTATACAGTTCGAACT	50.0	50.0	57.4	57.8	1451-1453 bp	1.0	75.4