Hotspot

petG-trnW-CCA

LSC · 66907-67035 · 129 bp

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Pi 0.0217

MarkerSeek score 64.00

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 129
variable_sites: 6
pi: 0.0217
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	CGTGGTGATCAATTGGACCT	AGAACGTGGGTCTCCAAAAC	50.0	50.0	57.9	58.0	197-206 bp	1.0	84.8
petG-trnW-CCA_p2	CGTGGTGATCAATTGGACCT	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.9	58.0	219-228 bp	1.0	84.4
petG-trnW-CCA_p3	ACGTGGTGATCAATTGGACC	AGAACGTGGGTCTCCAAAAC	50.0	50.0	58.2	58.0	198-207 bp	1.0	84.3
petG-trnW-CCA_p4	ACGTGGTGATCAATTGGACC	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.2	58.0	220-229 bp	1.0	83.9
petG-trnW-CCA_p5	CGACGTGGTGATCAATTGGA	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.3	58.0	222-231 bp	1.0	83.0