Hotspot

trnF-GAA-ndhJ

LSC · 49968-50684 · 717 bp

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Pi 0.019

MarkerSeek score 59.50

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 717
variable_sites: 34
pi: 0.019
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	TTTGCTCCAAGGAAGAACCC	55.0	50.0	58.0	58.0	1000-1010 bp	1.0	82.8
trnF-GAA-ndhJ_p2	TGTACGAACTGCTCAATCGG	TTTGCTCCAAGGAAGAACCC	50.0	50.0	58.0	58.0	1520-1529 bp	1.0	82.7
trnF-GAA-ndhJ_p3	GGGATAGCTCAGCTGGTAGA	GTGCGGATTTTCAAGAACGG	55.0	50.0	58.0	58.0	948-958 bp	1.0	82.7
trnF-GAA-ndhJ_p4	TGTACGAACTGCTCAATCGG	GTGCGGATTTTCAAGAACGG	50.0	50.0	58.0	58.0	1468-1477 bp	1.0	82.6
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	TGCTCCAAGGAAGAACCCTA	55.0	50.0	58.0	58.0	998-1008 bp	1.0	82.5