Hotspot

rps4-trnT-UGU

LSC · 47300-47879 · 580 bp

Back to Tamarix

Pi 0.00862

MarkerSeek score 59.40

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 580
variable_sites: 12
pi: 0.00862
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TCACTTCCACCTTTAGGCCT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.3	57.9	727-737 bp	1.0	79.1
rps4-trnT-UGU_p2	TCACTTCCACCTTTAGGCCT	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.3	57.8	695-705 bp	1.0	78.3
rps4-trnT-UGU_p3	CACTTCCACCTTTAGGCCTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.4	57.9	726-736 bp	1.0	76.4
rps4-trnT-UGU_p4	TCACTTCCACCTTTAGGCCT	ATCAGCCTGCTTAGCTCAGA	50.0	50.0	58.3	58.5	741-751 bp	1.0	76.0
rps4-trnT-UGU_p5	TCACTTCCACCTTTAGGCCT	CAGAGGTTAGAGCATCGCAT	50.0	50.0	58.3	57.5	725-735 bp	1.0	75.9