Hotspot

psbM-trnD-GUC

LSC · 29741-30664 · 924 bp

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Pi 0.012

MarkerSeek score 54.10

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 924
variable_sites: 27
pi: 0.012
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	CTGCTGATTCTAACCCGCTT	GGATTTGGCGGACAATTTGG	50.0	50.0	58.0	58.0	1363-1386 bp	1.0	83.1
psbM-trnD-GUC_p2	CTGCTGATTCTAACCCGCTT	TGGCGGACAATTTGGAAAGA	50.0	45.0	58.0	58.0	1358-1381 bp	1.0	83.1
psbM-trnD-GUC_p3	CTGCTGATTCTAACCCGCTT	CCGCCGATACGTGGTAATAG	50.0	55.0	58.0	58.0	1573-1596 bp	1.0	83.0
psbM-trnD-GUC_p4	CTGCTGATTCTAACCCGCTT	CATAGTATGATGGCGGTCGG	50.0	55.0	58.0	58.0	1875-1898 bp	1.0	82.8
psbM-trnD-GUC_p5	CTGCTGATTCTAACCCGCTT	ACATATGCCCCCATCGTCTA	50.0	50.0	58.0	58.0	1853-1876 bp	1.0	82.7