Hotspot

petN-psbM

LSC · 28856-29635 · 780 bp

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Pi 0.00513

MarkerSeek score 60.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 780
variable_sites: 10
pi: 0.00513
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	ACGTAAAAACGGTCAGCCAA	50.0	45.0	58.2	58.3	892-1042 bp	1.0	80.9
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	TACGTAAAAACGGTCAGCCA	50.0	45.0	58.2	57.5	893-1043 bp	1.0	79.4
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	ACGTAAAAACGGTCAGCCAA	50.0	45.0	57.6	58.3	831-981 bp	1.0	79.4
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	TACGTAAAAACGGTCAGCCA	50.0	45.0	57.6	57.5	832-982 bp	1.0	77.9
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	CGTAAAAACGGTCAGCCAAA	50.0	45.0	58.2	57.2	891-1041 bp	1.0	77.5