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trnC-GCA-petN

LSC · 28214-28765 · 552 bp

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Pi 0.0109

MarkerSeek score 59.90

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 552
variable_sites: 15
pi: 0.0109
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	ATTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.9	58.2	606-608 bp	1.0	79.9
trnC-GCA-petN_p2	ATTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	57.9	57.6	667-669 bp	1.0	78.1
trnC-GCA-petN_p3	TAAGGCGGAGGATTGCAAAT	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.6	58.2	631-633 bp	1.0	77.5
trnC-GCA-petN_p4	GTAAGGCGGAGGATTGCAAA	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.5	58.2	632-634 bp	1.0	76.6
trnC-GCA-petN_p5	TAAGGCGGAGGATTGCAAAT	AGAGTCCACTTCTTCCCCAT	45.0	50.0	57.6	57.6	692-694 bp	1.0	75.7