Hotspot

trnS-GCU-trnS-CGA

LSC · 8403-9306 · 904 bp

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Pi 0.0128
MarkerSeek score 67.50
Primer available yes
Label trnS-GCU-trnS-CGA

Feature Metadata

label_name
trnS-GCU-trnS-CGA
feature_type
igs
region
LSC
length_bp
904
variable_sites
27
pi
0.0128
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.97
markerseek_score
67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnS-CGA_p1 CCCTCGGTACAAATGACTCG ACCGAAAGACCCCTTAACTG 55.0 50.0 58.1 57.1 1016-1042 bp 1.0 77.2
trnS-GCU-trnS-CGA_p2 CCCTCGGTACAAATGACTCG CCGAAAGACCCCTTAACTGT 55.0 50.0 58.1 57.1 1015-1041 bp 1.0 77.2
trnS-GCU-trnS-CGA_p3 ACGGAAAGAGAGGGATTCGA ACCGAAAGACCCCTTAACTG 50.0 50.0 58.2 57.1 1037-1063 bp 1.0 76.6
trnS-GCU-trnS-CGA_p4 ACGGAAAGAGAGGGATTCGA CCGAAAGACCCCTTAACTGT 50.0 50.0 58.2 57.1 1036-1062 bp 1.0 76.6
trnS-GCU-trnS-CGA_p5 CCCTCGGTACAAATGACTCG CCGAAAGACCCCTTAACTGTT 55.0 47.6 58.1 57.9 1015-1041 bp 1.0 75.0