Hotspot

trnK-UUU-trnQ-UUG

LSC · 4234-7062 · 2829 bp

Back to Tamarix

Pi 0.0123

MarkerSeek score 61.60

Primer available yes

Label trnK-UUU-trnQ-UUG

Feature Metadata

label_name: trnK-UUU-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 2829
variable_sites: 72
pi: 0.0123
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-trnQ-UUG_p1	TCTACCGATGATGTGGACGA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	2557-2952 bp	1.0	85.2
trnK-UUU-trnQ-UUG_p2	AAAGCCGAGTACTCTACCGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	2492-2887 bp	1.0	84.3
trnK-UUU-trnQ-UUG_p3	AAGCCGAGTACTCTACCGTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	2491-2886 bp	1.0	84.3
trnK-UUU-trnQ-UUG_p4	TCAGTCGTGGTCTCACAAAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	2577-2972 bp	1.0	83.8
trnK-UUU-trnQ-UUG_p5	ACCGATGATGTGGACGAATC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	2554-2949 bp	1.0	83.8