Hotspot

trnL_uag

SSC · 120692-120771 · 80 bp

Back to Talipariti

Pi 0.0125

MarkerSeek score 59.70

Primer available yes

Label trnL_uag

Feature Metadata

label_name: trnL_uag
feature_type: tRNA
region: SSC
length_bp: 80
variable_sites: 1
pi: 0.0125
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL_uag_p1	TGAATTGACTTCAATCTCGACG	GAGGGTTTCTCGATTTTATGGA	40.9	40.9	56.8	55.9	219 bp	1.0	40.4
trnL_uag_p2	TGAATTGACTTCAATCTCGACGA	GAGGGTTTCTCGATTTTATGGA	39.1	40.9	58.2	55.9	219 bp	1.0	40.4
trnL_uag_p3	TGAATTGACTTCAATCTCGACG	GGGTTTCTCGATTTTATGGAATGG	40.9	41.7	56.8	58.2	217 bp	1.0	40.4
trnL_uag_p4	TGAATTGACTTCAATCTCGACGA	GGGTTTCTCGATTTTATGGAATGG	39.1	41.7	58.2	58.2	217 bp	1.0	40.4
trnL_uag_p5	GAATTGACTTCAATCTCGACGA	GAGGGTTTCTCGATTTTATGGA	40.9	40.9	56.6	55.9	218 bp	1.0	40.4