Hotspot

ycf1-ndhF

SSC · 115692-115967 · 276 bp

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Pi 0.0145

MarkerSeek score 60.80

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 276
variable_sites: 4
pi: 0.0145
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	TTTTTCGGAGGAAAGGGTGG	ATATGTAGGGGGAAGTCGCA	50.0	50.0	58.0	57.9	458-509 bp	1.0	79.8
ycf1-ndhF_p2	TTTTTCGGAGGAAAGGGTGG	TATGTAGGGGGAAGTCGCAT	50.0	50.0	58.0	57.9	457-508 bp	1.0	79.8
ycf1-ndhF_p3	GTGGATCCGAGCAAAATCGA	ATATGTAGGGGGAAGTCGCA	50.0	50.0	58.3	57.9	442-493 bp	1.0	77.1
ycf1-ndhF_p4	GTGGATCCGAGCAAAATCGA	TATGTAGGGGGAAGTCGCAT	50.0	50.0	58.3	57.9	441-492 bp	1.0	77.1
ycf1-ndhF_p5	TTTTTCGGAGGAAAGGGTGG	GGGAAGTCGCATCTCGTTTT	50.0	50.0	58.0	58.6	449-500 bp	1.0	76.1