Hotspot

atpB-rbcL

LSC · 58022-59147 · 1126 bp

Back to Talipariti

Pi 0.000903

MarkerSeek score 60.80

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 1126
variable_sites: 1
pi: 0.000903
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TTTCAAGCGTGGAAACTCCA	GGTGACATAAGTCCCTCCCT	45.0	55.0	58.0	58.1	1194-1204 bp	1.0	80.0
atpB-rbcL_p2	TTTCAAGCGTGGAAACTCCA	AACACCGGCTTTGAATCCAA	45.0	45.0	58.0	58.3	1237-1247 bp	1.0	78.4
atpB-rbcL_p3	TTTCAAGCGTGGAAACTCCA	TGGTGACATAAGTCCCTCCC	45.0	55.0	58.0	58.4	1195-1205 bp	1.0	77.5
atpB-rbcL_p4	TTTCAAGCGTGGAAACTCCA	TAACACCGGCTTTGAATCCA	45.0	45.0	58.0	57.4	1238-1248 bp	1.0	76.3
atpB-rbcL_p5	TTTCAAGCGTGGAAACTCCA	GTGGTGACATAAGTCCCTCC	45.0	55.0	58.0	57.3	1196-1206 bp	1.0	75.4