Hotspot

atpF-atpH

LSC · 13692-14303 · 612 bp

Back to Talipariti

Pi 0.00164

MarkerSeek score 60.20

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 612
variable_sites: 1
pi: 0.00164
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CAGTGACCCAAGGAAACGAA	TGGTTGTAGCATTGGCACTT	50.0	45.0	58.0	58.0	694-697 bp	1.0	80.1
atpF-atpH_p2	CAGTGACCCAAGGAAACGAA	CTGGTTGTAGCATTGGCACT	50.0	50.0	58.0	58.5	695-698 bp	1.0	76.5
atpF-atpH_p3	CAGTGACCCAAGGAAACGAA	ATGGGCTGGTTGTAGCATTG	50.0	50.0	58.0	58.5	700-703 bp	1.0	76.0
atpF-atpH_p4	AGTGACCCAAGGAAACGAAA	TGGTTGTAGCATTGGCACTT	45.0	45.0	57.3	58.0	693-696 bp	1.0	74.6
atpF-atpH_p5	CAGTGACCCAAGGAAACGAA	CCGAGGCAGAGGGAAAAATA	50.0	50.0	58.0	57.3	769-772 bp	1.0	74.4