Hotspot

trnS_gcu-trnG_ucc

LSC · 8546-9399 · 854 bp

Back to Talipariti

Pi 0

MarkerSeek score 62.80

Primer available yes

Label trnS_gcu-trnG_ucc

Feature Metadata

label_name: trnS_gcu-trnG_ucc
feature_type: igs
region: LSC
length_bp: 854
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS_gcu-trnG_ucc_p1	CAATCCGACGCTTTAGTCCA	ATGAATCAAACCGAGGGACC	50.0	50.0	58.0	57.6	903-969 bp	1.0	80.6
trnS_gcu-trnG_ucc_p2	AATCCGACGCTTTAGTCCAC	ATGAATCAAACCGAGGGACC	50.0	50.0	58.0	57.6	902-968 bp	1.0	80.6
trnS_gcu-trnG_ucc_p3	CGCACAACGGATTAGCAATC	ATGAATCAAACCGAGGGACC	50.0	50.0	57.9	57.6	918-984 bp	1.0	79.9
trnS_gcu-trnG_ucc_p4	ACGGAAAGAGAGGGATTCGA	ATGAATCAAACCGAGGGACC	50.0	50.0	58.2	57.6	957-1023 bp	1.0	79.4
trnS_gcu-trnG_ucc_p5	TCGCACAACGGATTAGCAAT	ATGAATCAAACCGAGGGACC	45.0	50.0	58.3	57.6	919-985 bp	1.0	78.5