Hotspot

psbH

LSC · 77078-77317 · 240 bp

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Pi 0

MarkerSeek score 24.10

Primer available yes

Label psbH

Feature Metadata

label_name: psbH
feature_type: gene
region: LSC
length_bp: 240
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 24.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH_p1	TTCAAGACCACAATGGATCT	CCTGTTCCTGGTTCTTTTGGA	40.0	47.6	54.2	58.1	352 bp	1.0	41.4
psbH_p2	TTCAAGACCACAATGGATCT	TCCTGTTCCTGGTTCTTTTGG	40.0	47.6	54.2	58.1	353 bp	1.0	41.4
psbH_p3	TTTCAAGACCACAATGGATCT	CCTGTTCCTGGTTCTTTTGGA	38.1	47.6	55.1	58.1	353 bp	1.0	40.4
psbH_p4	TTTCAAGACCACAATGGATCT	TCCTGTTCCTGGTTCTTTTGG	38.1	47.6	55.1	58.1	354 bp	1.0	40.4
psbH_p5	TCAAGACCACAATGGATCTATGA	CCTGTTCCTGGTTCTTTTGGA	39.1	47.6	57.0	58.1	351 bp	1.0	40.0