Hotspot

petD

LSC · 79889-80371 · 483 bp

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Pi 0.0104

MarkerSeek score 62.30

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 483
variable_sites: 5
pi: 0.0104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	CTCGAGCCGGATGATGAAAA	TCCCTAGATACACATGCTGTCT	50.0	45.5	58.1	58.1	598 bp	1.0	63.5
petD_p2	CTCGAGCCGGATGATGAAAA	ACTTTTGAAGCGACTATTCCCT	50.0	40.9	58.1	57.7	615 bp	1.0	61.9
petD_p3	TCGGGGGATGAATCGAATCT	TCCCTAGATACACATGCTGTCT	50.0	45.5	58.3	58.1	559 bp	1.0	61.8
petD_p4	CTCGAGCCGGATGATGAAAA	CACTTTTGAAGCGACTATTCCC	50.0	45.5	58.1	57.7	616 bp	1.0	61.4
petD_p5	CTTTCGGGGGATGAATCGAA	TCCCTAGATACACATGCTGTCT	50.0	45.5	57.7	58.1	562 bp	1.0	61.4