Hotspot

ccsA

SSC · 117400-118368 · 969 bp

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Pi 0.00206

MarkerSeek score 46.10

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 2
pi: 0.00206
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTGAAATCGGTAGACACGCT	GGCATGAGTGGTTTCATTGC	50.0	50.0	58.0	58.0	1720-1738 bp	1.0	81.5
ccsA_p2	TGAAATCGGTAGACACGCTG	GGCATGAGTGGTTTCATTGC	50.0	50.0	58.0	58.0	1719-1737 bp	1.0	81.5
ccsA_p3	GTGAAATCGGTAGACACGCT	TGAGTGGTTTCATTGCGGAA	50.0	45.0	58.0	58.0	1716-1734 bp	1.0	81.3
ccsA_p4	TGAAATCGGTAGACACGCTG	TGAGTGGTTTCATTGCGGAA	50.0	45.0	58.0	58.0	1715-1733 bp	1.0	81.3
ccsA_p5	CTTAGGAAGCAGTGCGAGAG	GGCATGAGTGGTTTCATTGC	55.0	50.0	58.1	58.0	1697-1715 bp	1.0	80.9