Hotspot

atpB-rbcL

LSC · 56149-56965 · 817 bp

Back to Talinum

Pi 0.00245
MarkerSeek score 60.20
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
817
variable_sites
2
pi
0.00245
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 CCTGGAGGAAAAGCTACGTC TCCAGGGGTTGATATTGAGGA 55.0 47.6 58.0 57.9 1028-1029 bp 1.0 70.9
atpB-rbcL_p2 CTGGAGGAAAAGCTACGTCC TCCAGGGGTTGATATTGAGGA 55.0 47.6 58.0 57.9 1027-1028 bp 1.0 70.9
atpB-rbcL_p3 GGAGGAAAAGCTACGTCCAG TCCAGGGGTTGATATTGAGGA 55.0 47.6 58.0 57.9 1025-1026 bp 1.0 70.9
atpB-rbcL_p4 AAACCCCAGGACCAGAAGTA TCCAGGGGTTGATATTGAGGA 50.0 47.6 57.9 57.9 952-953 bp 1.0 70.0
atpB-rbcL_p5 AACCCCAGGACCAGAAGTAG TCCAGGGGTTGATATTGAGGA 55.0 47.6 58.3 57.9 951-952 bp 1.0 68.3