Hotspot

trnF-GAA-ndhJ

LSC · 49524-50207 · 684 bp

Back to Talinum

Pi 0.00292

MarkerSeek score 60.40

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 684
variable_sites: 2
pi: 0.00292
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	826-827 bp	1.0	79.5
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	790-791 bp	1.0	79.1
trnF-GAA-ndhJ_p3	GGGATAGCTCAGCTGGTAGA	TCCACGCCTGAAACGTATTT	55.0	45.0	58.0	57.8	857-858 bp	1.0	78.8
trnF-GAA-ndhJ_p4	AAAGGGGAATGGTCGGGATA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	840-841 bp	1.0	78.7
trnF-GAA-ndhJ_p5	CTGGTAGAGCAGAGGACTGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	57.9	58.1	814-815 bp	1.0	78.4