Hotspot

trnS-GCU-trnT-CGU

LSC · 7959-8781 · 823 bp

Back to Talinum

Pi 0.00608

MarkerSeek score 58.70

Primer available yes

Label trnS-GCU-trnT-CGU

Feature Metadata

label_name: trnS-GCU-trnT-CGU
feature_type: igs
region: LSC
length_bp: 823
variable_sites: 5
pi: 0.00608
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnT-CGU_p1	ACGGAAAGAGAGGGATTCGA	GGGTTAGTAGAACGAATCGCA	50.0	47.6	58.2	57.8	957-963 bp	1.0	70.0
trnS-GCU-trnT-CGU_p2	ACGGAAAGAGAGGGATTCGA	ACGAATCGCACTTTTACCACT	50.0	42.9	58.2	58.2	946-952 bp	1.0	69.3
trnS-GCU-trnT-CGU_p3	ACGGAAAGAGAGGGATTCGA	ACGAATCGCACTTTTACCAC	50.0	45.0	58.2	56.8	946-952 bp	1.0	69.3
trnS-GCU-trnT-CGU_p4	ACGGAAAGAGAGGGATTCGA	AACGAATCGCACTTTTACCAC	50.0	42.9	58.2	57.5	947-953 bp	1.0	67.0
trnS-GCU-trnT-CGU_p5	ACGGAAAGAGAGGGATTCGA	GGGGTTAGTAGAACGAATCGC	50.0	52.4	58.2	58.5	958-964 bp	1.0	66.9