Hotspot

rps16-trnQ-UUG

LSC · 6014-6547 · 534 bp

Back to Talinum

Pi 0.00563
MarkerSeek score 60.60
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
534
variable_sites
3
pi
0.00563
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 607 bp 1.0 78.8
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 618 bp 1.0 78.3
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA TAAGGCATCGGGTTTTGGTC 45.0 50.0 58.1 58.2 637 bp 1.0 78.0
rps16-trnQ-UUG_p4 TCATGTCCTTCAAGTCGCAC TAAGGCATCGGGTTTTGGTC 50.0 50.0 58.2 58.2 648 bp 1.0 77.5
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA GTAAGGCATCGGGTTTTGGT 45.0 50.0 58.1 58.5 638 bp 1.0 75.7