Hotspot

trnK-UUU-rps16

LSC · 4117-4903 · 787 bp

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Pi 0.00254
MarkerSeek score 60.30
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
787
variable_sites
2
pi
0.00254
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GGTGCTCAACCTACGGAAAC 50.0 55.0 58.2 58.8 887-893 bp 1.0 72.2
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT GTGCTCAACCTACGGAAACC 50.0 55.0 58.2 58.8 886-892 bp 1.0 72.2
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT GGTGCTCAACCTACGGAAAC 50.0 55.0 58.2 58.8 886-892 bp 1.0 72.2
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT GTGCTCAACCTACGGAAACC 50.0 55.0 58.2 58.8 885-891 bp 1.0 72.2
trnK-UUU-rps16_p5 TCCTAGCCGCACTTAAAAGC GTGCTCAACCTACGGAAACC 50.0 55.0 58.3 58.8 901-907 bp 1.0 71.4