Hotspot

matK-trnK-UUU

LSC · 3386-4079 · 694 bp

Back to Talinum

Pi 0.00576

MarkerSeek score 62.10

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 694
variable_sites: 4
pi: 0.00576
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	AAGTTGTGTTGCCAAGACCT	TTGGGGGTTGCTAACTCAAC	45.0	50.0	57.9	58.0	788-789 bp	1.0	79.2
matK-trnK-UUU_p2	AAGTTGTGTTGCCAAGACCT	AACGGTAGAGTACTCGGCTT	45.0	50.0	57.9	58.2	771-772 bp	1.0	77.9
matK-trnK-UUU_p3	AAGTTGTGTTGCCAAGACCT	ACGGTAGAGTACTCGGCTTT	45.0	50.0	57.9	58.2	770-771 bp	1.0	77.9
matK-trnK-UUU_p4	AAGTTGTGTTGCCAAGACCT	GGGTTGCTAACTCAACGGTA	45.0	50.0	57.9	57.5	784-785 bp	1.0	75.6
matK-trnK-UUU_p5	AAGTTGTGTTGCCAAGACCT	TTTGGGGGTTGCTAACTCAA	45.0	45.0	57.9	57.2	789-790 bp	1.0	73.2