Hotspot

rps15-ycf1

SSC · 122574-122981 · 408 bp

Back to Takhtajaniantha

Pi 0.015
MarkerSeek score 52.50
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
408
variable_sites
6
pi
0.015
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 AGTCTCCGTATCTTATTGGTGA GAGTATGTTAAGGATATATATGTATCC 40.9 29.6 56.1 52.9 545-552 bp 1.0 41.9
rps15-ycf1_p2 AAGTCTCCGTATCTTATTGGTGA GAGTATGTTAAGGATATATATGTATCC 39.1 29.6 56.8 52.9 546-553 bp 1.0 41.9
rps15-ycf1_p3 AGTCTCCGTATCTTATTGGTGAA GAGTATGTTAAGGATATATATGTATCC 39.1 29.6 56.8 52.9 545-552 bp 1.0 41.9
rps15-ycf1_p4 GTCTCCGTATCTTATTGGTGA GAGTATGTTAAGGATATATATGTATCC 42.9 29.6 54.6 52.9 544-551 bp 1.0 41.9
rps15-ycf1_p5 AGTCTCCGTATCTTATTGGTG GAGTATGTTAAGGATATATATGTATCC 42.9 29.6 54.6 52.9 545-552 bp 1.0 41.9