Hotspot

ndhF-rpl32

SSC · 111338-112393 · 1056 bp

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Pi 0.0145

MarkerSeek score 60.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1056
variable_sites: 15
pi: 0.0145
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	CGGCCCAATATCCTTTCCTT	50.0	50.0	58.0	57.6	1228-1236 bp	1.0	79.4
ndhF-rpl32_p2	TGCCGTCGGAAAAAGAAGAA	CGGCCCAATATCCTTTCCTT	45.0	50.0	58.0	57.6	1226-1234 bp	1.0	79.3
ndhF-rpl32_p3	TTGCCGTCGGAAAAAGAAGA	CGGCCCAATATCCTTTCCTT	45.0	50.0	58.0	57.6	1227-1235 bp	1.0	79.3
ndhF-rpl32_p4	CTTTTTGTTGCCGTCGGAAA	CGGCCCAATATCCTTTCCTT	45.0	50.0	57.8	57.6	1234-1242 bp	1.0	78.1
ndhF-rpl32_p5	TTTGTTGCCGTCGGAAAAAG	CGGCCCAATATCCTTTCCTT	45.0	50.0	57.8	57.6	1231-1239 bp	1.0	78.1