Hotspot

rps16-trnQ-UUG

LSC · 6301-7323 · 1023 bp

Back to Takhtajaniantha

Pi 0.0154
MarkerSeek score 63.70
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1023
variable_sites
15
pi
0.0154
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1055-1096 bp 1.0 82.8
rps16-trnQ-UUG_p2 CGGATCGTGTCCTTCAAGTC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 1070-1111 bp 1.0 80.9
rps16-trnQ-UUG_p3 GGATCGTGTCCTTCAAGTCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 1069-1110 bp 1.0 80.9
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 1059-1100 bp 1.0 78.1
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA TAAGGCAACGGGTTTTGGTC 45.0 50.0 58.1 58.7 1085-1126 bp 1.0 77.9