Hotspot

rpoC1

Genome · 28574-31393 · 2820 bp

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Pi 0

MarkerSeek score 56.30

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: Genome
length_bp: 2820
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	TGCTCAGTCGAGAACTACGA	GGAAGGCTCCCTTGATCTCT	50.0	55.0	58.2	58.5	2986-2996 bp	1.0	74.8
rpoC1_p2	TGCTCAGTCGAGAACTACGA	CTGGAAGGCTCCCTTGATCT	50.0	55.0	58.2	58.8	2988-2998 bp	1.0	72.4
rpoC1_p3	TGCTCAGTCGAGAACTACGA	TGGAAGGCTCCCTTGATCTC	50.0	55.0	58.2	58.8	2987-2997 bp	1.0	72.4
rpoC1_p4	TTGCTCAGTCGAGAACTACG	GGAAGGCTCCCTTGATCTCT	50.0	55.0	57.4	58.5	2987-2997 bp	1.0	71.4
rpoC1_p5	TTGCTCAGTCGAGAACTACG	CTGGAAGGCTCCCTTGATCT	50.0	55.0	57.4	58.8	2989-2999 bp	1.0	69.0