Hotspot

clpP1

Genome · 116105-116782 · 678 bp

Back to Taiwania

Pi 0.00295

MarkerSeek score 60.40

Primer available yes

Label clpP1

Feature Metadata

label_name: clpP1
feature_type: gene
region: Genome
length_bp: 678
variable_sites: 2
pi: 0.00295
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1_p1	TATAGCATTCCTCGCGTTGG	GCTTATCAAAACCCGGTCCA	50.0	50.0	57.9	58.2	816 bp	1.0	77.6
clpP1_p2	TATAGCATTCCTCGCGTTGG	TGCTTATCAAAACCCGGTCC	50.0	50.0	57.9	58.2	817 bp	1.0	77.6
clpP1_p3	TATAGCATTCCTCGCGTTGG	TCAAAACCCGGTCCAAGAAA	50.0	45.0	57.9	57.6	811 bp	1.0	75.5
clpP1_p4	TATAGCATTCCTCGCGTTGG	ATCAAAACCCGGTCCAAGAA	50.0	45.0	57.9	57.3	812 bp	1.0	73.7
clpP1_p5	CACGAGGTGCTCCATTCTTA	GCTTATCAAAACCCGGTCCA	50.0	50.0	57.3	58.2	791 bp	1.0	73.5