Hotspot

atpA

Genome · 38975-40489 · 1515 bp

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Pi 0.00132

MarkerSeek score 59.50

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: Genome
length_bp: 1515
variable_sites: 2
pi: 0.00132
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	CGATCGAGCATAATATAAACCTGC	AGCTCTTGTATCCTGTTCCA	41.7	45.0	58.0	55.9	1689-1695 bp	1.0	40.1
atpA_p2	CGATCGAGCATAATATAAACCTGC	AGCTCTTGTATCCTGTTCCAA	41.7	42.9	58.0	56.6	1689-1695 bp	1.0	40.1
atpA_p3	CGATCGAGCATAATATAAACCTGC	AGCTCTTGTATCCTGTTCCAAA	41.7	40.9	58.0	57.4	1689-1695 bp	1.0	40.1
atpA_p4	CGATCGAGCATAATATAAACCTGC	CCATTGTGCTTGTAAATGTTGC	41.7	40.9	58.0	57.3	1724-1730 bp	1.0	40.1
atpA_p5	CGATCGAGCATAATATAAACCTGC	GCTCTTGTATCCTGTTCCAAAC	41.7	45.5	58.0	57.3	1688-1694 bp	1.0	40.1