Hotspot

ndhJ-chlN

Genome · 127142-128066 · 925 bp

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Pi 0

MarkerSeek score 58.90

Primer available yes

Label ndhJ-chlN

Feature Metadata

label_name: ndhJ-chlN
feature_type: igs
region: Genome
length_bp: 925
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-chlN_p1	GTTGGATTGGGTGGCCTTTA	ATTCATGGGTTTGCCAATGC	50.0	45.0	58.1	57.6	1106-1107 bp	1.0	76.3
ndhJ-chlN_p2	TTGGATTGGGTGGCCTTTAC	ATTCATGGGTTTGCCAATGC	50.0	45.0	58.1	57.6	1105-1106 bp	1.0	76.3
ndhJ-chlN_p3	GTTGGATTGGGTGGCCTTTA	CCAGATTCATGGGTTTGCCA	50.0	50.0	58.1	58.4	1110-1111 bp	1.0	76.0
ndhJ-chlN_p4	TTGGATTGGGTGGCCTTTAC	CCAGATTCATGGGTTTGCCA	50.0	50.0	58.1	58.4	1109-1110 bp	1.0	76.0
ndhJ-chlN_p5	GCCTGAAAGTTGGATTGGGT	ATTCATGGGTTTGCCAATGC	50.0	45.0	58.4	57.6	1114-1115 bp	1.0	73.9