Hotspot

clpP1-accD

Genome · 116783-117224 · 442 bp

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Pi 0.00226

MarkerSeek score 42.70

Primer available yes

Label clpP1-accD

Feature Metadata

label_name: clpP1-accD
feature_type: igs
region: Genome
length_bp: 442
variable_sites: 1
pi: 0.00226
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1-accD_p1	CTCTGCATCGAAGGCTGATT	TTGTCGCACTCGGAGTTATG	50.0	50.0	58.0	58.0	1310-1311 bp	1.0	79.6
clpP1-accD_p2	TCTGCATCGAAGGCTGATTC	TTGTCGCACTCGGAGTTATG	50.0	50.0	58.1	58.0	1309-1310 bp	1.0	79.6
clpP1-accD_p3	ACCTTAGGGATGGGGTTTGA	TTGTCGCACTCGGAGTTATG	50.0	50.0	57.9	58.0	898-899 bp	1.0	79.5
clpP1-accD_p4	ACTCCCTCTGTCATTCCTCC	TTGTCGCACTCGGAGTTATG	55.0	50.0	58.1	58.0	1105-1106 bp	1.0	79.0
clpP1-accD_p5	CTCCCTCTGTCATTCCTCCA	TTGTCGCACTCGGAGTTATG	55.0	50.0	58.1	58.0	1104-1105 bp	1.0	79.0