Hotspot

ccsA-clpP1

Genome · 115575-116104 · 530 bp

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Pi 0

MarkerSeek score 26.20

Primer available yes

Label ccsA-clpP1

Feature Metadata

label_name: ccsA-clpP1
feature_type: igs
region: Genome
length_bp: 530
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 26.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-clpP1_p1	GGGTTACACAGCTATGGGTG	AAAGATGATTTGTTTTCTGTGT	55.0	27.3	58.0	52.8	584 bp	1.0	40.0
ccsA-clpP1_p2	GGTTACACAGCTATGGGTGG	AAAGATGATTTGTTTTCTGTGT	55.0	27.3	58.0	52.8	583 bp	1.0	40.0
ccsA-clpP1_p3	GAAAGGGGATAAACCGGCAA	AAAGATGATTTGTTTTCTGTGT	50.0	27.3	58.2	52.8	669 bp	1.0	40.0
ccsA-clpP1_p4	GGGTTACACAGCTATGGGTG	AAAAGATGATTTGTTTTCTGTGT	55.0	26.1	58.0	53.6	585 bp	1.0	40.0
ccsA-clpP1_p5	GGTTACACAGCTATGGGTGG	AAAAGATGATTTGTTTTCTGTGT	55.0	26.1	58.0	53.6	584 bp	1.0	40.0