Hotspot

petD-petB

Genome · 90510-90732 · 223 bp

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Pi 0

MarkerSeek score 56.90

Primer available yes

Label petD-petB

Feature Metadata

label_name: petD-petB
feature_type: igs
region: Genome
length_bp: 223
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-petB_p1	GATCTGCACAGCCTAACCAA	TGCACTTTCTAATGATCCGCA	50.0	42.9	57.9	58.0	298-313 bp	1.0	74.9
petD-petB_p2	TTGATCTGCACAGCCTAACC	TGCACTTTCTAATGATCCGCA	50.0	42.9	57.9	58.0	300-315 bp	1.0	74.9
petD-petB_p3	GATCTGCACAGCCTAACCAA	CCGCAAACAAGGTATTTCAGG	50.0	47.6	57.9	57.8	282-297 bp	1.0	73.4
petD-petB_p4	TTGATCTGCACAGCCTAACC	CCGCAAACAAGGTATTTCAGG	50.0	47.6	57.9	57.8	284-299 bp	1.0	73.4
petD-petB_p5	GTTCAAGTCACACACTCCCA	TGCACTTTCTAATGATCCGCA	50.0	42.9	57.7	58.0	276-291 bp	1.0	73.3