Hotspot

trnF-GAA-ndhD

Genome · 44961-45235 · 275 bp

Back to Taiwania

Pi 0.00364

MarkerSeek score 54.60

Primer available yes

Label trnF-GAA-ndhD

Feature Metadata

label_name: trnF-GAA-ndhD
feature_type: igs
region: Genome
length_bp: 275
variable_sites: 1
pi: 0.00364
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhD_p1	GCTAGTATGATGCACAGGGG	AGGTATTGGTCTTTACCCCGA	55.0	47.6	57.8	58.2	460 bp	1.0	69.5
trnF-GAA-ndhD_p2	GCTAGTATGATGCACAGGGG	GGTATTGGTCTTTACCCCGA	55.0	50.0	57.8	56.6	459 bp	1.0	67.7
trnF-GAA-ndhD_p3	GCTAGTATGATGCACAGGGG	AGGTATTGGTCTTTACCCCG	55.0	50.0	57.8	56.6	460 bp	1.0	67.7
trnF-GAA-ndhD_p4	GCTAGTATGATGCACAGGGG	TGGTCTTTACCCCGATCTAGT	55.0	47.6	57.8	57.6	454 bp	1.0	67.6
trnF-GAA-ndhD_p5	GTATGATGCACAGGGGAACA	AGGTATTGGTCTTTACCCCGA	50.0	47.6	57.6	58.2	456 bp	1.0	67.5