Hotspot

psaC-ndhE

SSC · 120118-120595 · 478 bp

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Pi 0.0286
MarkerSeek score 60.70
Primer available yes
Label psaC-ndhE

Feature Metadata

label_name
psaC-ndhE
feature_type
igs
region
SSC
length_bp
478
variable_sites
19
pi
0.0286
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ndhE_p1 GGACAAGCGGATTCACATCT ACAAGCCGAAGCATGGTTAA 50.0 45.0 58.0 58.1 894-937 bp 0.666667 67.0
psaC-ndhE_p2 AGCTCGAACACATTGGGTAC ACAAGCCGAAGCATGGTTAA 50.0 45.0 57.9 58.1 778-821 bp 0.666667 66.6
psaC-ndhE_p3 TGAAGAACCCGCGAATATGG ACAAGCCGAAGCATGGTTAA 50.0 45.0 58.1 58.1 1156-1199 bp 0.666667 66.5
psaC-ndhE_p4 CTGGCCAAGCTGCTAAAGTA ACAAGCCGAAGCATGGTTAA 50.0 45.0 57.9 58.1 1415-1458 bp 0.666667 65.8
psaC-ndhE_p5 TGGCCAAGCTGCTAAAGTAG ACAAGCCGAAGCATGGTTAA 50.0 45.0 57.9 58.1 1414-1457 bp 0.666667 65.8