Hotspot

rpl32-trnL-UAG

SSC · 116058-116746 · 689 bp

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Pi 0.0136
MarkerSeek score 65.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
689
variable_sites
14
pi
0.0136
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TCCACCGGACAGTCAAAAAG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.0 57.9 762-788 bp 1.0 84.1
rpl32-trnL-UAG_p2 TCCACCGGACAGTCAAAAAG AGCAGCGTGTCTACCAATTT 50.0 45.0 58.0 57.8 755-781 bp 1.0 83.7
rpl32-trnL-UAG_p3 TCCACCGGACAGTCAAAAAG CCTAAGAGCAGCGTGTCTAC 50.0 55.0 58.0 57.8 761-787 bp 1.0 83.6
rpl32-trnL-UAG_p4 TCCACCGGACAGTCAAAAAG CTAAGAGCAGCGTGTCTACC 50.0 55.0 58.0 57.8 760-786 bp 1.0 83.6
rpl32-trnL-UAG_p5 TCCACCGGACAGTCAAAAAG GAGCAGCGTGTCTACCAATT 50.0 50.0 58.0 58.3 756-782 bp 1.0 83.0