Hotspot

rps8-rpl14

LSC · 83024-83222 · 199 bp

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Pi 0.0335
MarkerSeek score 69.60
Primer available yes
Label rps8-rpl14

Feature Metadata

label_name
rps8-rpl14
feature_type
igs
region
LSC
length_bp
199
variable_sites
10
pi
0.0335
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps8-rpl14_p1 CGGCAATAATGTCCCTACCC AACTCGAATCTTTGGTGCGA 55.0 45.0 58.1 57.8 306-357 bp 1.0 80.5
rps8-rpl14_p2 CGGCAATAATGTCCCTACCC CTCGGGAATTGCGACAATTG 55.0 50.0 58.1 57.8 283-334 bp 1.0 80.5
rps8-rpl14_p3 CGGCAATAATGTCCCTACCC TCGGGAATTGCGACAATTGA 55.0 45.0 58.1 58.2 282-333 bp 1.0 80.5
rps8-rpl14_p4 CGGCAATAATGTCCCTACCC ACTCGAATCTTTGGTGCGAT 55.0 45.0 58.1 57.6 305-356 bp 1.0 78.7
rps8-rpl14_p5 CGGCAATAATGTCCCTACCC CGAATCTTTGGTGCGATTGC 55.0 50.0 58.1 58.5 302-353 bp 1.0 78.1