Hotspot

psbB-psbT

LSC · 76031-76500 · 470 bp

Back to Tainia

Pi 0.025
MarkerSeek score 75.70
Primer available yes
Label psbB-psbT

Feature Metadata

label_name
psbB-psbT
feature_type
igs
region
LSC
length_bp
470
variable_sites
17
pi
0.025
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbB-psbT_p1 TCAGAGATGTTTTTGCCGGT TGGAACTTTAGGTGGTTCTCG 45.0 47.6 57.7 57.6 664-766 bp 1.0 70.9
psbB-psbT_p2 TCAGAGATGTTTTTGCCGGT GGAACTTTAGGTGGTTCTCGA 45.0 47.6 57.7 57.3 663-765 bp 1.0 68.7
psbB-psbT_p3 GCCAGAACCTTGTTCAGAGA TGGAACTTTAGGTGGTTCTCG 50.0 47.6 57.4 57.6 677-779 bp 1.0 68.6
psbB-psbT_p4 GCTCAAGTGGAATTTGGAGC TGGAACTTTAGGTGGTTCTCG 50.0 47.6 57.4 57.6 626-728 bp 1.0 68.2
psbB-psbT_p5 TGGAGATCCAACTACGAGGA TGGAACTTTAGGTGGTTCTCG 50.0 47.6 57.2 57.6 594-696 bp 1.0 67.0