Hotspot

clpP-psbB

LSC · 73744-74503 · 760 bp

Back to Tainia

Pi 0.028
MarkerSeek score 79.40
Primer available yes
Label clpP-psbB

Feature Metadata

label_name
clpP-psbB
feature_type
igs
region
LSC
length_bp
760
variable_sites
31
pi
0.028
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP-psbB_p1 CGCACTATACGTCAACCCAA GGCCCAACCAGAAACTAGAG 50.0 55.0 58.0 57.9 911-938 bp 1.0 85.5
clpP-psbB_p2 TATACGTCAACCCAAGCTGC GGCCCAACCAGAAACTAGAG 50.0 55.0 58.0 57.9 906-933 bp 1.0 85.4
clpP-psbB_p3 CTTTTGGAACACCAATGGGC GGCCCAACCAGAAACTAGAG 50.0 55.0 57.8 57.9 857-884 bp 1.0 84.6
clpP-psbB_p4 TTTGGAACACCAATGGGCAT GGCCCAACCAGAAACTAGAG 45.0 55.0 58.3 57.9 855-882 bp 1.0 83.7
clpP-psbB_p5 TCCAGGACTCCGAAAAGGTA GGCCCAACCAGAAACTAGAG 50.0 55.0 57.7 57.9 877-904 bp 1.0 83.3