Hotspot

ndhC-trnV-UAC

LSC · 51888-52244 · 357 bp

Back to Tainia

Pi 0.0962
MarkerSeek score 82.60
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
357
variable_sites
51
pi
0.0962
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TAGGATTGGGTTGGTCAGGT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 421-453 bp 1.0 90.0
ndhC-trnV-UAC_p2 TTGGGTTGGTCAGGTTGAAG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 416-448 bp 1.0 89.9
ndhC-trnV-UAC_p3 TGCGACTTAGGATTGGGTTG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 428-460 bp 1.0 89.6
ndhC-trnV-UAC_p4 GATTGGGTTGGTCAGGTTGA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 418-450 bp 1.0 88.1
ndhC-trnV-UAC_p5 TAGGATTGGGTTGGTCAGGT AGCCCAATGCAACAAAATGC 50.0 45.0 57.9 58.5 503-535 bp 1.0 87.7