Hotspot

rpoB-trnC-GCA

LSC · 27396-28813 · 1418 bp

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Pi 0.0186
MarkerSeek score 59.60
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1418
variable_sites
39
pi
0.0186
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 AATTCTTCCGTCAAGCCCTG CCTTACCACTTGGCCATGC 50.0 57.9 58.2 58.8 1532-1545 bp 1.0 67.6
rpoB-trnC-GCA_p2 AATTCTTCCGTCAAGCCCTG GCCTTACCACTTGGCCATG 50.0 57.9 58.2 58.8 1533-1546 bp 1.0 67.6
rpoB-trnC-GCA_p3 AAATTCTTCCGTCAAGCCCT CCTTACCACTTGGCCATGC 45.0 57.9 57.4 58.8 1533-1546 bp 1.0 64.3
rpoB-trnC-GCA_p4 AAATTCTTCCGTCAAGCCCT GCCTTACCACTTGGCCATG 45.0 57.9 57.4 58.8 1534-1547 bp 1.0 64.3
rpoB-trnC-GCA_p5 CTTCCGTCAAGCCCTGATTA CCTTACCACTTGGCCATGC 50.0 57.9 57.3 58.8 1528-1541 bp 1.0 63.5